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人α突触核蛋白(α-SYN)ELISA试剂盒

试剂盒

市场价:￥1320.00

价格:￥0.00

<p><span style=";font-size:14px;font-family:SimSun,Bold;color:rgb(0,0,0);font-weight:bold">检测范围： </span><span style=";font-size:21px;font-family:TimesNewRomanPS-BoldMT;color:rgb(0,0,0);font-weight:bold">96T </span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">6ng/L -220 ng/L </span><span style=";font-size:14px;font-family:SimSun,Bold;color:rgb(0,0,0);font-weight:bold">使用目的： </span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">本试剂盒用于测定小鼠血清、血浆及相关液体样本中 α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">突触核蛋白</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">(</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-SYN)</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">含量。 </span><span style=";font-size:14px;font-family:SimSun,Bold;color:rgb(0,0,0);font-weight:bold">实验原理 </span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">本试剂盒应用双抗体夹心法测定标本中小鼠 α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">突触核蛋白</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">(</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-SYN)</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">水平。用纯化的 </span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">小鼠 α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">突触核蛋白</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">(</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-SYN)</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次 </span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">加入 α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">突触核蛋白</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">(</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-SYN)</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">，再与 </span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">HRP </span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">标记的 α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">突触核蛋白</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">(</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-SYN)</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">抗体结合，形成 </span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">抗体</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">抗原</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">酶标抗体复合物，经过彻底洗涤后加底物 </span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">TMB </span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">显色。</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">TMB </span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">在 </span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">HRP </span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">酶的催化下 </span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的 α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">突触核蛋白 </span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">(</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-SYN)</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">呈正相关。用酶标仪在 </span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">450nm </span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">波长下测定吸光度（</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">OD </span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">值），通过标准曲线计算样 </span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">品中小鼠 α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">突触核蛋白</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">(</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">α</span><span style=";font-size:14px;font-family:TimesNewRomanPSMT;color:rgb(0,0,0)">-SYN)</span><span style=";font-size:14px;font-family:宋体;color:rgb(0,0,0)">浓度。</span></p>
猫三碘甲状腺原氨酸(T3)ELISA试剂盒

试剂盒

市场价:￥0.00

价格:￥1390.00

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